Comparison of Two Different PCR-based Methods for Detection of GAA Expansions in Frataxin Gene

Mona ENTEZA­M, Akbar AMIRFIROOZI, Mansoureh TOGHA, Mohammad KERAMATIPOUR; Iranian Journal of Public Health 2017. 46(2):222-228.

The presence or absence of expanded alleles can be identified correctly by TP-PCR. Performing long-PCR and Fluorescent-long-PCR enables accurate genotyping in all samples. This approach is highly reliable. It could be successfully used for detection of GAA expansion repeats.


 Comparison of Two Different PCR-based Methods for Detection of GAA Expansions in Frataxin Gene