Selecting and isolating colonies of human induced pluripotent stem cells reprogrammed from adult fibroblasts.

J Vis Exp. 2012 Feb 20;(60). pii: 3416. doi: 10.3791/3416.

Polak U, Hirsch C, Ku S, Gottesfeld J, Dent SY, Napierala M.
Department of Molecular Carcinogenesis and Center for Cancer Epigenetics, University of Texas M.D. Anderson Cancer Center.

Keywords: human adult fibroblasts, human induced pluripotent stem cells (hiPSC), retroviral vectors, Oct3/4, Sox2, Klf4, c-myc (OSKM), Friedreich ataxia.