The Iron−Sulfur Cluster of Pyruvate Formate-Lyase Activating Enzyme in Whole Cells: Cluster Interconversion and a Valence-Localized [4Fe-4S]2+ State

Biochemistry, Article ASAP

DOI: 10.1021/bi9010286

Publication Date (Web): August 27, 2009

Jian Yang‡, Sunil G. Naik§, Danilo O. Ortillo§, Ricardo Garca-Serres§, Meng Li‡, William E. Broderick, Boi Hanh Huynh*§ and Joan B. Broderick*

Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59718

^‡ Department of Chemistry, Michigan State University, East Lansing, Michigan 48824

^§ Department of Physics, Emory University, Atlanta, Georgia 30322

KEYWORDS: Pyruvate formate-lyase activating enzyme (PFL-AE),  glycyl radical, pyruvate formate-lyase (PFL),  [4Fe-4S] cluster, cluster interconversions,  [4Fe-4S]²⁺  , [2Fe-2S]²⁺ , Fe^III,  Fe^II,  redox state, 5′-deoxyadenosine, AMP, ADP, and methylthioadenosine, oxidative damage.