Our results showed that despite the self-destruction of the CjCas9 gene, an effective genome editing of the FXN gene was obtained in vitro. CRISPR-SCReT (Stop Codon Read Through), we inserted a stop codon (TGA) at the beginning of the CjCas9 gene to repress its expression. Subsequently, we induced the expression of Cas9 by molecules capable to allow translation despite the presence of the premature stop codon.
Tuesday, January 5, 2021
Two ways to control CjCas9 expression in the deletion of pathogenic GAA repeat in frataxin gene
Pouiré Yaméogo; Genetic Engineering Research, Vol.9 Iss.3:sc165
Subscribe to:
Posts (Atom)
