Dörte Poburski, Josefine Barbara Boerner, Michel Koenig, Michael Ristow, René Thierbach. Biology Open 2016 : doi: 10.1242/bio.017004
OPEN ACCESS
Here, we have developed a new mammalian cell model employing the Cre/loxP recombination system to induce a homozygous or heterozygous frataxin knockout in mouse embryonic fibroblasts. Induction of Cre-mediated disruption by tamoxifen was successfully tested on RNA and protein levels. The robustness of the newly established system may additionally be used for a time-resolved study of pharmacological candidates in a HTS manner.
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