Interactions of iron-bound frataxin with ISCU and ferredoxin on the cysteine desulfurase complex leading to Fe-S cluster assembly

Kai Cai, Ronnie O. Frederick, Marco Tonelli, John L. Markley, Journal of Inorganic Biochemistry, Volume 183, 2018, Pages 107-116,
ISSN 0162-0134, doi: 10.1016/j.jinorgbio.2018.03.007.

Frataxin (FXN) binds one Fe2+; the Fe2+-FXN complex is stabilized by its interaction with the scaffold protein (ISCU) on cysteine desulfurase. Upon the addition of reduced ferredoxin (Red-FDX) and l-cysteine, ferredoxin is oxidized (Ox-FDX), l-cysteine is converted to l-alanine generating sulfur, Fe2+ is oxidized to Fe3+, Fe-S binds to ISCU.