Biophysical characterisation of the recombinant human frataxin precursor

Ignacio Hugo Castro, Alejandro Ferrari, María Georgina Herrera, Martín Ezequiel Noguera, Lorenzo Maso, Monica Benini, Alessandra Rufini, Roberto Testi, Paola Costantini and Javier Santos; FEBS Open Bio (2018). Accepted Article. DOI: 10.1002/2211-5463.12376

We investigated the conformation, stability and function of a recombinant precursor variant (His6-TAT-FXN1-210), which includes a TAT peptide in the N-terminal region to assist with transport across cell membranes. His6-TAT-FXN1-210 was expressed in E. coli and conditions were found for purifying folded protein free of aggregation, oxidation or degradation, even after freezing and thawing.

Biophysical characterisation of the recombinant human frataxin precursor