Rapid DNA Methylation Analysis of the FXN gene in Friedreich

Rapid DNA Methylation Analysis of the FXN gene in Friedreich. S. Al-Mahdawi, C. Sandi, M. A. Pook; Poster 488T. American Society of Human Genetics (ASHG) 2013 Annual Meeting, Octubre, 22-26, Boston.

KEYWORDS: Friedreich ataxia (FRDA), frataxin, epigenetic changes, heterochromatin formation, DNA methylation, GAA repeat, histone acetylation and methylation, specific ‘methylscreen’ restriction enzyme digestion, qPCR-based protocols,. to more rapidly quantify DNA methylation at four of the upstream CpG sites, rapid detection of DNA methylation.