Evidence That Phosphorylation of Iron Regulatory Protein 1 at Serine 138 Destabilizes the [4Fe-4S] Cluster in Cytosolic Aconitase by Enhancing 4Fe-3Fe

Originally published In Press as doi:10.1074/jbc.M807717200 on March 6, 2009 J. Biol. Chem., Vol. 284, Issue 19, 12701-12709, May 8, 2009

Evidence That Phosphorylation of Iron Regulatory Protein 1 at Serine 138 Destabilizes the [4Fe-4S] Cluster in Cytosolic Aconitase by Enhancing 4Fe-3Fe Cycling^*

Kathryn M. Deck, Aparna Vasanthakumar, Sheila A. Anderson, Jeremy B. Goforth¹, M. Claire Kennedy, William E. Antholine^¶, and Richard S. Eisenstein²

From the Department of Nutritional Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706, the Biochemistry Department, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, and the ^¶National Biomedical ESR Center, Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-3548

Received for publication, October 6, 2008 , and in revised form, March 5, 2009.

This paper is dedicated to the memory of Helmut Beinert.

^* This work was supported in part by National Institutes of Health Grants DK 66600 (to R. S. E.) and National Biomedical ESR Center Grant EB001980. This work was also supported by United States Department of Agriculture Cooperative States Research Education and Extension Service Grant 2006-35200-16604 and University of Wisconsin-Madison Hatch Project 4885 (to R. S. E.).

The on-line version of this article (available at http://www.jbc.org/) contains supplemental Figs. S1 and S2.

¹ Supported by National Institutes of Health Training Grant T32 DK007665.

² To whom correspondence should be addressed: University of Wisconsin, Dept. of Nutritional Sciences, 1415 Linden Dr., Madison, WI 53706. Tel.: 608-262-5830; Fax: 608-262-5860; E-mail: eisenste@nutrisci.wisc.edu
.