Evaluation of antibodies for western blot analysis of frataxin protein isoforms

Liwei Weng, Qingqing Wang, Sixiang Yu, Xiaolu Yang, David R. Lynch, Clementina Mesaros, Ian A. Blair; Journal of Immunological Methods,
2019, doi:10.1016/j.jim.2019.07.001.

Well-validated antibodies are required for use in western blot analysis to determine whether levels of various forms of frataxin have been increased. Here we examined the specificity of five commercially available anti-frataxin antibodies and determined whether they detect mature frataxin in mouse heart tissue. Four protein standards of monkey, human, and mouse frataxin as well as mouse heart tissue were examined using polyacrylamide gel electrophoresis (PAGE) in combination with western blot analysis.



Evaluation of antibodies for western blot analysis of frataxin protein isoforms